Consulta: creatorFacets:"Roela,R.A."
Registros recuperados: 2
Data/hora: 19/05/2024 00:53:44

Differential regulation of vitamin D receptor expression in distinct leukemic cell lines upon phorbol ester-induced growth arrest
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Folgueira,M.A.A.K.; Federico,M.H.H.; Roela,R.A.; Maistro,S.; Katayama,M.L.H.; Brentani,M.M.
A close correlation between vitamin D receptor (VDR) abundance and cell proliferation rate has been shown in NIH-3T3 fibroblasts, MCF-7 breast cancer and in HL-60 myeloblastic cells. We have now determined if this association occurs in other leukemic cell lines, U937 and K562, and if VDR content is related to c-myc expression, which is also linked to cell growth state. Upon phorbol myristate acetate (PMA) treatment, cells from the three lineages (HL-60, U937 and K562) differentiated and expressed specific surface antigens. All cell lines analyzed were growth inhibited by PMA and the doubling time was increased, mainly due to an increased fraction of cells in the G0/G1 phase, as determined by flow cytometry measurements of incorporated bromodeoxyuridine and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Receptors; Calcitriol; Cell division; Cell differentiation; Leukemia; Phorbol esters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500011

Simultaneous changes in the function and expression of beta 1 integrins during the growth arrest of poorly differentiated colorectal cells (LISP-1)
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Roela,R.A.; Brentani,M.M.; Katayama,M.L.H.; Reis,M.; Federico,M.H.H.
Cells usually lose adhesion and increase proliferation and migration during malignant transformation. Here, we studied how proliferation can affect the other two characteristics, which ultimately lead to invasion and metastasis. We determined the expression of ß1 integrins, as well as adhesion and migration towards laminin-1, fibronectin, collagens type I and type IV presented by LISP-1 colorectal cancer cells exposed to 2.5% dimethyl sulfoxide (DMSO), an agent capable of decreasing proliferation in this poorly differentiated colorectal cell line. Untreated cells (control), as shown by flow cytometry and monoclonal antibodies, expressed alpha2 (63.8 ± 11.3% positive cells), alpha3 (93.3 ± 7.0%), alpha5 (50.4 ± 12.0%) and alpha6 (34.1 ± 4.9%) integrins but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion molecules; Cell migration; Integrin; Cell cycle; DMSO.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800016